GLUCOSE
Synonyms - Specimen - Reference Value - Method Collection of sample preservation of samples - Description
Indications of Blood Glucose Determination
Synonyms : Blood sugar , Fasting blood sugar (FBS)
specimen : Serum (1 mL) , plasma (1 mL)Collected in( sodium fluoride)
Reference Value : 80 - 120 mg/dl , renal threshold 140 - 170 mg/dl
Method : Spectrophotometry
Collection of samples
- Blood specimens should be collected after an over night fast ( usually 12 hours ) to prevent influences of the diet on blood glucose .
- All collection equipments must be sterile and chemically clean .
Preservation of samples
1 - Sodium fluoride is used as anticoagulant and preservative
2 - Refrigeration at 4 degree
3 - Freezing ( analysis should be performed 2 - 3 hours after removal from freezer ) . some damage to enzymes from freezing may occurred , but activity returned at room temperature .
Freezing and thawing should not be permitted more than once before analysis because it may be denature the constituents .Glucose level decrease at rate of 10% / hour at room temperature.
Description ( Glucose )
Glucose , a six carbon sugar ( monosaccharide ) , enters the diet as a part of sugars sucrose , lactose and maltose and as major constituents of complex poly saccharides called dietary starch .
The body acquires most of its energy from the oxidative metabolism of glucose . Excess glucose is stored in the liver or in muscle tissue as glycogen .
Diabetes is a disease characterized by hyperglycemia or elevated glucose levels .
Indications of Blood Glucose Determination
1 - Diabetes mellitus or pencreatitis
2 - Increase of unexplained comas and convulsions
3 - As a routine physical examination